Reference datasets of tufA and UPA markers to identify algae in metabarcoding surveys

Abstract

The data presented here are related to the research article “Multi-marker metabarcoding of coral skeletons reveals a rich microbiome and diverse evolutionary origins of endolithic algae” (Marcelino and Verbruggen, 2016) [1]. Here we provide reference datasets of the elongation factor Tu (tufA) and the Universal Plastid Amplicon (UPA) markers in a format that is ready-to-use in the QIIME pipeline (Caporaso et al., 2010) [2]. In addition to sequences previously available in GenBank, we included newly discovered endolithic algae lineages using both amplicon sequencing (Marcelino and Verbruggen, 2016) [1] and chloroplast genome data (Marcelino et al., 2016; Verbruggen et al., in press) [3,4]. We..